Journal
ASIAN JOURNAL OF ORGANIC CHEMISTRY
Volume 4, Issue 8, Pages 724-728Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/ajoc.201500116
Keywords
carbohydrates; fluorescent probes; lectins; photoaffinity labeling; photocrosslinking
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Funding
- JSPS [25560400]
- Ajinomoto Scholarship
- Grants-in-Aid for Scientific Research [25560400] Funding Source: KAKEN
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A set of lactose-based photoaffinity probes bearing either alkyl diazirine or trifluoromethylphenyl diazirine (TPD) groups were designed and synthesized to directly compare their efficiency in photocrosslinking a carbohydrate-binding protein. The crosslinking efficiency of the TPD probes was higher than the alkyl diazirine probes when reacted with a single binding protein. However, the alkyl diazirine probe with a small alkyne tag achieved more selective photoaffinity labeling of a binding protein in cell lysate than the corresponding TPD probe.
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