Journal
TROPICAL ANIMAL HEALTH AND PRODUCTION
Volume 42, Issue 6, Pages 1271-1275Publisher
SPRINGER
DOI: 10.1007/s11250-010-9560-z
Keywords
Camel pox; Virus isolation; Identification; Characterization; PCR; Sequence analysis
Funding
- Ministry of Forest and Environment (MOFE), Government of India
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In this study, we isolated and identified three camel pox viruses (CMLV) from two outbreaks of camel pox infection in camels associated with eruptions on cheeks, nostrils, limbs, scrotum, and sheath that occurred at different places of Bikaner district, Rajasthan (India). The scab specimens collected were subjected for virus isolation in Vero cell culture, and the isolated viruses were characterized by employing polymerase chain reaction (PCR) and sequencing. The causative agent was identified as CMLV, based on A-type inclusion, B5R and C18L genes-specific PCRs and partial sequencing of these genes, which clearly confirmed that the outbreaks were caused by CMLV and identity of CMLV isolates. Further, phylogenetic analysis of partial C18L gene sequences have showed that Indian CMLV are clustered together with other reported isolates/strains.
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