Journal
TRENDS IN PLANT SCIENCE
Volume 18, Issue 9, Pages 473-476Publisher
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tplants.2013.07.003
Keywords
transgenes; CaMV35S promoter; GFP fusions; protein localization
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Fluorescent protein (FP) fusions are frequently used to localize and follow the movement of proteins in living cells. However, a consensus is missing about the experimental design and controls that guarantee the reliability of the results. Here, we discuss possible artifacts and try to navigate through the many methods, preferences, and assumptions that surround protein localization in plants that make it difficult to design a universal approach to achieve reliable results.
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