Journal
TRENDS IN MOLECULAR MEDICINE
Volume 19, Issue 5, Pages 273-280Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.molmed.2013.01.004
Keywords
stem cells; mutation rate; stem cells variability; clinical application; biosafety
Funding
- Russian Presidential Program 'Leading Scientific Schools' [5638.2010.4]
- Presidium RAS Program 'Molecular and Cell Biology'
- Federal target program [16.512.12.2002]
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Stem cells (SCs) are thought to have great therapeutic potential, but due to continuously and stochastically arising new mutations that unpredictably change the composition of a cell population, the large-scale manufacturing of SCs with uniform properties and predictable behavior is a challenge. Quantitative evaluation of the characteristic mutation rate of a given stem cell line could be an important criterion in making the decision to use the line in medical practice. Such an evaluation could provide a new quality standard for newly derived human embryonic stem cell (hESC) lines prior to depositing them in stem cell banks. Here, we substantiate this view with simple calculations showing the effect of the mutation rate on changes in the cell population composition due to amplification. Selection of SCs with low mutation rate could reduce the risk of negative side effects during treatment.
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