Journal
TRENDS IN MICROBIOLOGY
Volume 18, Issue 9, Pages 397-405Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.tim.2010.06.004
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Funding
- National Foundation for Scientific Research (FNRS)
- Foundation for Training in Industrial and Agricultural Research (FRIA)
- Universite Catholique de Louvain (Fonds Speciaux de Recherche)
- Region wallonne
- Federal Office for Scientific, Technical and Cultural Affairs
- Research Department of the Communaute francaise de Belgique
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How cell envelope constituents are spatially organised and how they interact with the environment are key questions in microbiology. Unlike other bioimaging tools, atomic force microscopy (AFM) provides information about the nanoscale surface architecture of living cells and about the localization and interactions of their individual constituents. These past years have witnessed remarkable advances in our use of the AFM molecular toolbox to observe and force probe microbial cells. Recent milestones include the real-time imaging of the nanoscale organization of cell walls, the quantification of subcellular chemical heterogeneities, the mapping and functional analysis of individual cell wall constituents and the analysis of the mechanical properties of single receptors and sensors.
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