Journal
TRENDS IN MICROBIOLOGY
Volume 16, Issue 2, Pages 73-79Publisher
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tim.2007.12.001
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Funding
- Biotechnology and Biological Sciences Research Council [BBS/B/13799] Funding Source: researchfish
- Biotechnology and Biological Sciences Research Council [BBS/B/13799] Funding Source: Medline
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Bacillus subtilis and its close relatives are widely used for the production of enzymes for the detergent, food and beverage industries. These organisms not only produce an appropriate range of enzymes but also have the capacity to secrete them into the culture medium at high concentrations. Purification from the culture medium rather than from the cytoplasm considerably reduces downstream processing costs. In recent years, considerable effort has been aimed at developing B. subtilis as a host for the production of heterologous proteins. The folded state of the target protein at various stages of the secretion pathway has proved to be important.
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