Journal
TRENDS IN BIOTECHNOLOGY
Volume 32, Issue 10, Pages 506-512Publisher
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tibtech.2014.08.001
Keywords
protein engineering; nanobiotechnology; mechanobiology; synthetic biology; supramolecular assembly; biomimetic
Categories
Funding
- Medical Research Council
- Merton College Oxford
- Clarendon Fund
- Oxford University Department of Biochemistry
- St. Peter's College, Oxford
- European Research Council [ERC-2013-CoG 615945-PeptidePadlock]
- Medical Research Council [1243569] Funding Source: researchfish
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Biotechnology is often limited by weak interactions. We suggest that an ideal interaction between proteins would be covalent, specific, require addition of only a peptide tag to the protein of interest, and form under a wide range of conditions. Here we summarize peptide tags that are able to form spontaneous amide bonds, based on harnessing reactions of adhesion proteins from the bacterium Streptococcus pyogenes. These include the irreversible peptide-protein interaction of SpyTag with SpyCatcher, as well as irreversible peptide-peptide interactions via SpyLigase. We describe existing applications, including polymerization to enhance cancer cell capture, assembly of living biomaterial, access to diverse protein shapes, and improved enzyme resilience. We also indicate future opportunities for resisting biological force and extending the scope of protein nanotechnology.
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