Journal
TRENDS IN BIOTECHNOLOGY
Volume 30, Issue 1, Pages 8-16Publisher
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tibtech.2011.08.002
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Funding
- National Heart Lung and Blood Institute, National Institutes of Health
- National Research Council (NRC) (NIST)
- National Institute of Allergy and Infectious Diseases, National Institutes of Health
- NIST
- National Institute for Biomedical Imaging and Bioengineering of the NIH
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Watching biological molecules provides clues to their function and regulation. Some of the most powerful methods of labeling proteins for imaging use genetically encoded fluorescent fusion tags. There are four standard genetic methods of covalently tagging a protein with a fluorescent probe for cellular imaging. These use (i) autofluorescent proteins, (ii) self-labeling enzymes, WO enzymes that catalyze the attachment of a probe to a target sequence, and (iv) biarsenical dyes that target tetracysteine motifs. Each of these techniques has advantages and disadvantages. In this review, we cover new developments in these methods and discuss practical considerations for their use in imaging proteins inside living cells.
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