Journal
TRENDS IN BIOTECHNOLOGY
Volume 29, Issue 6, Pages 276-283Publisher
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tibtech.2011.01.010
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Funding
- Fondo di Ateneo per la Ricerca (University of Insubria)
- MIUR [2008N2FM4A]
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D-Amino acid oxidase (DAAO) is a well-known flavoenzyme that catalyzes the oxygen-dependent oxidative de-amination of amino acid D-isomers with absolute stereospecificity, which results in a-keto acids, ammonia and hydrogen peroxide. Recently, the extraordinary functional plasticity of DAAO has become evident; in turn, boosting research on this flavoprotein. Protein engineering has allowed for a redesign of DAAO substrate specificity, oxygen affinity, cofactor binding, stability, and oligomeric state. We review recent developments in utilizing DAAO, including as a biocatalyst for resolving racemic amino acid mixtures, as a tool for biosensing, and as a new mechanism of herbicide resistance. Perspectives for future biotechnological applications of this oxidative biocatalyst are also outlined.
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