Journal
TRENDS IN BIOTECHNOLOGY
Volume 26, Issue 1, Pages 48-54Publisher
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tibtech.2007.10.007
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Funding
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM066712] Funding Source: NIH RePORTER
- NIGMS NIH HHS [GM66712] Funding Source: Medline
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Enzymes are highly selective catalysts that perform intricate chemistries at ambient temperatures and pressures. Although water is the solvent of life, it is a poor solvent for most synthetic organic reactions and, therefore, most chemists avoid aqueous solutions for synthetic applications. However, when removed from the aqueous environment and placed in an organic solvent, enzyme activity is reduced greatly. Here, we present a general overview of recent efforts to activate enzymes for use in nonaqueous media, giving particular focus to the use of simple salts as additives that result in significant biocatalytic improvements.
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