Journal
TRENDS IN BIOCHEMICAL SCIENCES
Volume 38, Issue 5, Pages 263-271Publisher
ELSEVIER SCIENCE LONDON
DOI: 10.1016/j.tibs.2013.02.008
Keywords
RNA interference; Argonaute; slicing; structure and function
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Funding
- Jane Coffin Childs Memorial Fund for Medical Research
- Louis Morin Charitable Trust
- Robertson Research Fund of Cold Spring Harbor Laboratory
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Despite the fact that different classes of small RNAs are generated by largely different biogenesis pathways, all mature small RNAs associate with an Argonaute family member to form the RNA-induced silencing complex (RISC). Gene silencing by RISC could not be studied in molecular detail because structural information on eukaryotic Argonautes was lacking. Recently, however, the structure of human Argonaute-2 (hAgo2), a model for RISC function, was determined in complexes with heterogeneous guide RNA and in complexes with a specific miRNA. We review the exciting advances that these two structures, together with the structure of a budding yeast Argonaute, brought to the field of eukaryotic RNA interference (RNAi), and how they will enable a more detailed mechanistic understanding of eukaryotic RISC.
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