Journal
TREES-STRUCTURE AND FUNCTION
Volume 24, Issue 6, Pages 975-992Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00468-010-0488-8
Keywords
Cuttings; Gymnosperms; In vitro culture; Micropropagation; Microshoots
Categories
Funding
- FCT Portugal [PTDC/AGR-CFL/71437/2006]
- Fundação para a Ciência e a Tecnologia [PTDC/AGR-CFL/71437/2006] Funding Source: FCT
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In conifers, vegetative propagation of superior genotypes is the most direct means for making large genetic gains, because it allows a large proportion of genetic diversity to be captured in a single cycle of selection. There are two aims of vegetative propagation, namely large-scale multiplication of select genotypes and production of large numbers of plants from scarce and costly seed that originates from controlled seed orchard pollinations. This can be achieved, in some species, either through rooted cuttings or rooted microshoots, the latter regenerated through tissue culture in vitro. Thus far, both strategies have been used but often achieved limited success mainly because of difficult and inefficient rooting process. In this overview of technology, we focus on the progress in defining the physical and chemical factors that help the conifer cuttings and microshoots to develop adventitious roots. These factors include plant growth regulators, carbohydrates, light quality, temperature and rooting substrates/media as major variables for development of reliable adventitious rooting protocols for different conifer species.
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