Journal
TREES-STRUCTURE AND FUNCTION
Volume 24, Issue 4, Pages 597-607Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00468-010-0430-0
Keywords
ATPase; NaCl; Populus euphratica; Populus popularis; Respiratory rate; Salt tolerance; Western blotting; X-ray microanalysis
Categories
Funding
- HI-TECH Research and Development Program of China [2006AA10Z131]
- National Natural Science Foundation of China [30430430, 30872005]
- Foundation for the Supervisors of Beijing Excellent Doctoral Dissertation [YB20081002201]
- National Excellent Doctoral Dissertation of PR China [200152]
- Teaching and Research Award Program for Outstanding Young Teachers in Higher Education Institution of Ministry of Education (MOE), Ministry of Education (PRC) [2002-323]
- Key project of MOE [2009-84]
- Natural Science Foundation of Hubei province [2007ABB003]
- PLIE (the Platform of Large Instruments and Equipment) at the Beijing Forestry University
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During a 30-day period of increasing salinity, we examined the effects of NaCl on leaf H+-ATPase and salinity tolerance in 1-year-old plants of Populus euphratica Oliv. (salt resistant) and P. popularis 35-44 (P. popularis) (salt sensitive). Electron probe X-ray microanalysis of leaf mesophyll revealed that P. euphratica had a higher ability to retain lower NaCl concentrations in the cytoplasm, as compared to P. popularis. The sustained activity of H+ pumps (by cytochemical staining) in salinised P. euphratica suggests a role in energising salt transport through the plasma membrane (PM) and tonoplast. Salt-induced alterations of leaf respiration, ATP content and expression of PM H+-ATPase were compared between the two species. Results show that P. euphratica retained a constant respiratory rate, ATP production and protein abundance of PM H+-ATPase (by Western blotting) in salt-stressed plants. P. euphratica was able to maintain a comparatively high capacity of ATP hydrolysis and H+ pumping during prolonged salt exposure. By contrast, the activity and expression of PM H+-ATPase were markedly decreased in P. popularis leaves in response to salt stress. Furthermore, NaCl-stressed P. popularis plants showed a marked decline of respiration (70%) and ATP production (66%) on day 30. We conclude that the inability of P. popularis to transport salt to the apoplast and vacuole was partly due to the decreased activity of H+ pumps. As a consequence, cytosolic ion concentrations were observed to be comparatively high for an extended period of time, so that cell metabolism, in particular respiration, was disrupted in P. popularis leaves.
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