Journal
TREE PHYSIOLOGY
Volume 28, Issue 6, Pages 921-927Publisher
OXFORD UNIV PRESS
DOI: 10.1093/treephys/28.6.921
Keywords
carbon chain elongation; fatty acid synthesis; seed oil
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A cDNA clone encoding a putative beta-ketoacylacyl carrier protein (ACP) synthase III (KAS III ) was isolated from Jatropha curcas L., a woody oil plant. The cDNA clone (named JcKAS III) contained a 1203-bp open reading frame coding for 400 amino acids with a predicted molecular mass of about 42 kDa. The deduced amino acid sequence of the cDNA clone shares about 80% identity to KAS III from other plants, and contains a conserved Cys(176) in the active site and the amino acid motif G(355) NTSAAS(361) which is responsible for binding regulatory acyl-ACPs. Southern blotting analysis indicated that JcKAS III is a single copy gene in the J. curcas genome. Quantitative real-time PCR analysis showed that JcKAS III was expressed in all tissues examined with highest expression in roots, and that expression of JcKAS III increased as seeds developed.
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