Journal
TREE GENETICS & GENOMES
Volume 8, Issue 1, Pages 177-194Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s11295-011-0431-1
Keywords
Xyloglucan endotransglycosylase/ hydrolases (XTHs); Gene duplications and divergence; Gene expression; Populus; Arabidopsis
Categories
Funding
- Department of Energy-Bioenergy Center
- Consortium for Plant Biotechnology Research, Inc.
- Office of Biological and Environmental Research in the Department of Energy Office of Science
Ask authors/readers for more resources
Xyloglucan endotransglycosylase/hydrolases (XTHs) are believed to modify the cell wall structure by cleaving a xyloglucan polymer and transferring the newly generated, potentially reducing, terminal to another xyloglucan. We report here the detailed analysis of 37 Populus trichocarpa XTH genes/proteins in their divergence in both the coding and 5' promoter regions. Our results show that the Populus XTH genes have experienced whole-genome and local duplications and pre- and post-speciation divergence. Genome-wide and segmental duplications seem to be dominant in subfamily I and III, while tandem duplication seems to be the major mechanism for the subfamily II expansion, which also has higher average ratios of K (a)/K (s) compared to those in subfamily I and III. There was a general lack of organ-specific gene expression. In contrast, the expression patterns in subfamily II varied in response to various hormone treatments, with II-A being up-regulated and II-B down-regulated after 2 h of hormone treatment. Expression for this subfamily was verified using the 1.5-kb PtXTH22 promoter that was fused with the GUS reporter gene and transformed into Arabidopsis. The PtXTH22 promoter contains auxin response element, ethylene insensitive 3-like factors, and brassinosteroid response cis-elements. Histochemical GUS staining of transgenic Arabidopsis seedlings confirmed that the PtXTH22 promoter was up-regulated by several hormones.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available