Journal
TRANSPLANTATION PROCEEDINGS
Volume 40, Issue 8, Pages 2661-2664Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.transproceed.2008.08.062
Keywords
-
Categories
Funding
- National Natural Science Foundation of China [30572273]
- Natural Science foundation of Jiangsu [06KJD310185]
- Ministry of Health (L.Z.), Jiangsu, China
Ask authors/readers for more resources
Objective. We investigated the degree and the time course of endothelial injury in mice pretreated with lethal or reduced-intensity irradiation administered before transplantation. Materials and Methods. Six- to eight-week-old female mice were randomly allocated into three groups: lethal-intensity irradiation (8.5 Gy, group 1), reduced-intensity irradiation (5,0 Gy, group 2), or nonirradiated controls (group 3). After conditioning, circulating endothelial cells (CD31(+), CD133(-), and CD45(low)) and peripheral blood CD4(+) or CD8(+) T-lymphocyte subpopulations were enumerated using flow cytometry at various times. The morphologic changes in endothelium were examined at phase-contrast light microscopy. Results. Circulating endothelial cells showed an earlier and higher peak in the lethal irradiation group compared with the reduced-intensity Irradiation group, Which exhibited a protean elevation in cell numbers. There were no visible histopathologic changes during the early stage of endothelial damage. Conclusions. Lethal and reduced doses of irradiation induced endothelial injury ill a dose-dependent manner. Endothelial damage may occur before graft-vs-host disease and its related complications.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available