Journal
TOXINS
Volume 7, Issue 12, Pages 5377-5385Publisher
MDPI AG
DOI: 10.3390/toxins7124883
Keywords
aptamer; Ochratoxin A (OTA); gold nanoparticles; cationic polymer; colorimetric assay
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Funding
- National Science Foundation of China [41301350]
- Innovation and Capacity-building Projects by Beijing Academy of Agriculture and Forestry Sciences [KJCX20140302]
- National Key Technology R&D Program of the Ministry of Science and Technology [2014BAD04B05-2]
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A label-free aptamer-based assay for the highly sensitive and specific detection of Ochratoxin A (OTA) was developed using a cationic polymer and gold nanoparticles (AuNPs). The OTA aptamer was used as a recognition element for the colorimetric detection of OTA based on the aggregation of AuNPs by the cationic polymer. By spectroscopic quantitative analysis, the colorimetric assay could detect OTA down to 0.009 ng/mL with high selectivity in the presence of other interfering toxins. This study offers a new alternative in visual detection methods that is rapid and sensitive for OTA detection.
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