Journal
STEM CELLS TRANSLATIONAL MEDICINE
Volume 5, Issue 2, Pages 175-185Publisher
WILEY
DOI: 10.5966/sctm.2015-0080
Keywords
Rotary suspension culture; Megakaryopoiesis; Thrombopoiesis; CD34(+) cell
Categories
Funding
- National Basic Research Program of China [2012CB966403, 2015CB964902, 2011CB710905]
- Chinese National Natural Science Foundation [31171431, 31471291]
- Basic Scientific Research Funds in National Nonprofit Institutes [744]
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Platelet transfusion has been widely used in patients undergoing chemotherapy or radiotherapy; however, the shortage of the platelet supply limits the care of patients. Although derivation of clinical-scale platelets in vitro could provide a new source for transfusion, the devices and procedures for deriving scalable platelets for clinical applications have not been established. In the present study, we found that a rotary cell culture system (RCCS) can potentiate megakaryopoiesis and significantly improve the efficiency of platelet generation. When used with chemical compounds and growth factors identified via small-scale screening, the RCCS improved platelet generation efficiency by as much as similar to 3.7-fold compared with static conditions. Shear force, simulated microgravity, and better diffusion of nutrients and oxygen from the RCCS, altogether, might account for the improved efficient platelet generation. The cost-effective and highly controllable strategy and methodology represent an important step toward large-scale platelet production for future biomedical and clinical applications.
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