Journal
TRANSPLANT INTERNATIONAL
Volume 24, Issue 8, Pages 856-864Publisher
WILEY
DOI: 10.1111/j.1432-2277.2011.01275.x
Keywords
beta-cell regeneration; beta-cell replication; exendin-4; GLP-1; islet transplantation
Categories
Funding
- JDRF
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Exendin-4 can stimulate beta-cell replication in mice. Whether it can stimulate beta-cell replication in human islet grafts remains unknown. Therefore, we compared the effects of exendin-4 on beta-cell replication in mouse and human islet grafts. Islets, isolated from mouse and human donors at different ages, were transplanted into diabetic mice and/or diabetic nude mice that were given bromodeoxyuridine ( BrdU) with or without exendin-4. At 4 weeks post-transplantation, islet grafts were removed for insulin and BrdU staining and quantification of insulin(+)/BrdU(+) cells. Although diabetes was reversed in all mice transplanting syngeneic mouse islets from young or old donors, normoglycemia was achieved significantly faster in exendin-4 treated mice. Mouse islet grafts in exendin-4 treated mice had significantly more insulin (+)/BrdU(+) beta cells than in untreated mice (P < 0.01). Human islet grafts from <= 22-year-old donors had more insulin (+)/BrdU(+) beta cells in exendin-4 treated mice than that in untreated mice (P < 0.01). However, human islet grafts from >= 35-year-old donors contained few insulin (+)/BrdU(+) beta cells in exendin-4 treated or untreated mice. Our data demonstrated that the capacity for beta-cell replication in mouse and human islet grafts is different with and without exendin-4 treatment and indicated that GLP-1 agonists can stimulate beta-cell replication in human islets from young donors.
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