Journal
TRANSGENIC RESEARCH
Volume 23, Issue 1, Pages 177-185Publisher
SPRINGER
DOI: 10.1007/s11248-013-9764-x
Keywords
Mouse; Genetics; Transgenics; Mammalian; Cre recombinase
Categories
Funding
- Wellcome Trust [WT098051]
- NIH [U42RR033192]
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We describe here use of a cell-permeable Cre to efficiently convert the EUCOMM/KOMP-CSD tm1a allele to the tm1b form in preimplantation mouse embryos in a high-throughput manner, consistent with the requirements of the International Mouse Phenotyping Consortium-affiliated NIH KOMP2 project. This method results in rapid allele conversion and minimizes the use of experimental animals when compared to conventional Cre transgenic mouse breeding, resulting in a significant reduction in costs and time with increased welfare benefits.
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