Journal
TRANSGENIC RESEARCH
Volume 22, Issue 1, Pages 195-200Publisher
SPRINGER
DOI: 10.1007/s11248-012-9635-x
Keywords
ES cells; C57BL/6N; Transgenic mice; EGFP; ICSI
Categories
Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- Grants-in-Aid for Scientific Research [20670006, 24700430, 21112006, 20062008] Funding Source: KAKEN
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Occasionally, chimeras do not transmit the gene of interest to pups in gene disruption experiments. However, the risk of failure could be reduced if we could identify embryonic stem (ES)-derived germ cells in the testis. Here, we report the production of pups from three lines of infertile chimeric male mice and the establishment of knockout lines by combining green fluorescent protein-tagged ES cells with intracytoplasmic sperm injection.
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