Journal
TRANSGENIC RESEARCH
Volume 20, Issue 6, Pages 1285-1292Publisher
SPRINGER
DOI: 10.1007/s11248-011-9495-9
Keywords
E8 promoter; Miraculin; Taste-modifying protein; Tomato
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Funding
- Ministry of Economy, Trade, and Industry of Japan
- Grants-in-Aid for Scientific Research [23880001] Funding Source: KAKEN
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The E8 promoter, a tomato fruit-ripening-specific promoter, and the CaMV 35S promoter, a constitutive promoter, were used to express the miraculin gene encoding the taste-modifying protein in tomato. The accumulation of miraculin protein and mRNA was compared among transgenic tomatoes expressing the miraculin gene driven by these promoters. Recombinant miraculin protein predominantly accumulated in transgenic tomato lines using the E8 promoter (E8-MIR) only at the red fruit stage. The accumulations were almost uniform among all fruit tissues. When the 35S promoter (35S-MIR) was used, miraculin accumulation in the exocarp was much higher than in other tissues, indicating that the miraculin accumulation pattern can be regulated by using different types of promoters. We also discuss the potential of the E8-MIR lines for practical use.
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