Journal
TRANSGENIC RESEARCH
Volume 20, Issue 3, Pages 665-673Publisher
SPRINGER
DOI: 10.1007/s11248-010-9450-1
Keywords
RNA interference (RNAi); Helicoverpa armigera; Transgenic; Gossypium hirsutum; Insect-proof
Categories
Funding
- National Science Foundation of China [30870380]
- National High-Tech Research Program of China [2009AA 10Z112]
- Ministry of Agriculture of China [2008ZX 08009-001]
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RNA interference (RNAi) plays an important role in regulating gene expression in eukaryotes. Previously, we generated Arabidopsis and tobacco plants expressing double-stranded RNA (dsRNA) targeting a cotton bollworm (Helicoverpa armigera) P450 gene, CYP6AE14. Bollworms fed on transgenic dsCYP6AE14 plants showed suppressed CYP6AE14 expression and reduced growth on gossypol-containing diet (Mao et al., in Nat Biotechnol 25: 1307-1313, 2007). Here we report generation and analysis of dsRNA-expressing cotton (Gossypium hirsutum) plants. Bollworm larvae reared on T2 plants of the ds6-3 line exhibited drastically retarded growth, and the transgenic plants were less damaged by bollworms than the control. Quantitative reverse-transcription polymerase chain reaction (RT-PCR) showed that the CYP6AE14 expression level was reduced in the larvae as early as 4 h after feeding on the transgenic plants; accordingly, the CYP6AE14 protein level dropped. These results demonstrated that transgenic cotton plants expressing dsCYP6AE14 acquired enhanced resistance to cotton bollworms, and that RNAi technology can be used for engineering insect-proof cotton cultivar.
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