Journal
TRANSGENIC RESEARCH
Volume 19, Issue 6, Pages 959-975Publisher
SPRINGER
DOI: 10.1007/s11248-010-9374-9
Keywords
Cotton; Disease resistance; NPR1; Reniform nematodes; Seedling diseases; Transgenic cotton; Wilt diseases
Categories
Funding
- Cotton Inc.
- Texas Higher Education Coordinating Board-Advanced Research Program [000517-0005-2006]
- Texas AgriLife Research
- Division Of Integrative Organismal Systems
- Direct For Biological Sciences [0827200] Funding Source: National Science Foundation
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Cotton is an economically important crop worldwide that suffers severe losses due to a wide range of fungal/bacterial pathogens and nematodes. Given its susceptibility to various pathogens, it is important to obtain a broad-spectrum resistance in cotton. Resistance to several fungal and bacterial diseases has been obtained by overexpressing the Non-expressor of Pathogenesis-Related genes-1 (NPR1) in various plant species with apparently minimal or no pleiotropic effects. We examined the efficacy of this approach in cotton by constitutive expression of the Arabidopsis (Arabidopsis thaliana) NPR1 gene. The results show that NPR1-expressing lines exhibited significant resistance to Verticillium dahliae isolate TS2, Fusarium oxysporum f. sp. vasinfectum, Rhizoctonia solani, and Alternaria alternata. Interestingly, the transformants also showed significant resistance to reniform nematodes. Analysis of defense-related, biochemical and molecular responses suggest that when challenged with pathogens or certain systemic acquired resistance-inducing chemicals, the transgenic lines respond to a greater degree compared to the wildtype plants. Importantly, the basal activities of the defense-related genes and enzymes in uninduced transformants were no different than those in their non-transgenic counterparts. The results provide additional evidence supporting the role of NPR1 as an important part of the plant defense system and suggest a means to achieve broad-spectrum resistance to pathogens via genetic engineering.
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