Journal
TRANSGENIC RESEARCH
Volume 19, Issue 4, Pages 637-645Publisher
SPRINGER
DOI: 10.1007/s11248-009-9347-z
Keywords
GADD34; Wound healing; Cell migration; Myosin
Categories
Funding
- Ministry of Education, Science, Technology, Sports and Culture, Japan
- Grants-in-Aid for Scientific Research [22700691] Funding Source: KAKEN
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Wound healing consists of sequential steps of tissue repair, and cell migration is particularly important. In order to analyze the potential function of growth arrest and DNA damage inducible protein 34 (GADD34) in tissue repair, we performed in vitro and in vivo wound healing experiments. In an in vitro scratch assay, GADD34 knockout (KO) mouse embryonic fibroblasts (MEFs) had higher migration rates than did wild type (WT) MEFs. Furthermore, the rate of wound closure was faster in GADD34 KO MEFs than in WT MEFs. Using in vivo punch biopsy assays, GADD34 KO mice had accelerated wound healing compared to WT mice. WT mice expressed higher amounts of myosin IIA in migrating macrophages and myofibroblasts than did GADD34 KO mice. These results indicate that GADD34 negatively regulates cell migration in wound healing via expression of myosin IIA.
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