Journal
TRANSGENIC RESEARCH
Volume 18, Issue 2, Pages 185-192Publisher
SPRINGER
DOI: 10.1007/s11248-008-9205-4
Keywords
Transgenic rice; Oral vaccine; Ascaris suum; Cholera toxin B subunit; Mucosal adjuvant
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Funding
- Ministry of Education, Culture, Sports, Science, and Technology, Japan [16380198, 19658113]
- Bio-oriented Technology Research Advancement Institution (BRAIN)
- Program of Founding Research Centers for Emerging and Reemerging Infectious Diseases, Ministry of Education, Culture, Sports, Science, and Technology, Japan
- Grants-in-Aid for Scientific Research [16380198, 19658113] Funding Source: KAKEN
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Cereal crops such as maize and rice are considered attractive for vaccine production and oral delivery. Here, we evaluated the rice Oryza sativa for production of As16-an antigen protective against the roundworm Ascaris suum. The antigen was produced as a chimeric protein fused with cholera toxin B subunit (CTB), and its expression level in the endosperm reached 50 mu g/g seed. Feeding the transgenic (Tg) rice seeds to mice elicited an As16-specific serum antibody response when administered in combination with cholera toxin (CT) as the mucosal adjuvant. Although omitting the adjuvant from the vaccine formulation resulted in failure to develop the specific immune response, subcutaneous booster immunization with bacterially expressed As16 induced the antibody response, indicating priming capability of the Tg rice. Tg rice/CT-fed mice orally administered A. suum eggs had a lower lung worm burden than control mice. This suggests that the rice-delivered antigen functions as a prophylactic edible vaccine for controlling parasitic infection in animals.
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