Journal
TRANSACTIONS OF THE AMERICAN FISHERIES SOCIETY
Volume 137, Issue 5, Pages 1292-1298Publisher
WILEY
DOI: 10.1577/T07-190.1
Keywords
-
Categories
Funding
- Montana Fish, Wildlife, and Parks
Ask authors/readers for more resources
Native inland populations of rainbow trout Oncorhynchus mykiss gairdneri, particularly resident populations, often hybridize with introduced populations of the widely cultured coastal form of the species, O. m. irideus. The inland and coastal subspecies differ genetically from each other by allozyme polymorphisms at the lactate dehydrogenase (LDH-B2*) and superoxide dismutase loci (sSOD-1*) that can be detected using protein electrophoresis. Fewer laboratories, however, are now using allozyme technology, and most genetic studies from wild organisms are now being conducted using DNA rather than protein analyses. We have identified the single-nucleotide polymorphism (SNP) differences responsible for the protein variations by sequencing the complementary DNA for the LDH-B2* and sSOD-1* genes in a large number of individuals whose genotypes were also determined by protein electrophoresis. The genetic differences causing the allozyme polymorphisms have been converted into SNP allelic discrimination assays. This should allow simple, efficient tests to be conducted in a large number of laboratories as an aid to assessing the level of hybridization between inland and coastal rainbow trout. It should also allow DNA studies to be more directly related to previous allozyme studies. High variability was also found at other sites in the superoxide dismutase gene.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available