Journal
TRAFFIC
Volume 10, Issue 11, Pages 1579-1584Publisher
WILEY
DOI: 10.1111/j.1600-0854.2009.00980.x
Keywords
lipid droplets; live cell microscopy; 3T3-L1 adipocytes; HuH7 hepatoma cells
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Funding
- National Academic Foundation
- EC
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A lipophilic dye based on the Bodipy fluorophore, LD540, was developed for microscopic imaging of lipid droplets. In contrast to previous lipid droplet dyes, it can spectrally be resolved from both green and red fluorophores allowing multicolor imaging in both fixed and living cells. Its improved specificity, brightness and photostability support live cell imaging, which was used to demonstrate by two-color imaging lipid droplet motility along microtubules.
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