Journal
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
Volume 63, Issue -, Pages 129-139Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.trac.2014.06.021
Keywords
Drug-drug interaction; In-vitro drug-metabolism; Mass spectrometry (MS); Metabolic stability; Metabolite identification; Metabolite profiling; Reactive metabolite; Sub-2 mu m particle; Ultra-high-pressure liquid chromatography (UHPLC); UHPLC-MS
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This review emphasizes the benefits of ultra-high-pressure liquid chromatography (UHPLC) in the context of in-vitro drug-metabolism and pharmacokinetics (DMPK) studies. The analysis throughput afforded by UHPLC is particularly useful during the early DMPK stages, when rapid, reliable data must be generated on a large number of new chemical entities (NCEs) to select the most appropriate drug candidates. UHPLC also offers high chromatographic resolution, an important feature for the identification of unknown metabolites that are potentially generated from NCEs by a few dozen biochemical enzymes and combinations of them. Most DMPK studies require selectivity and peak-assignment certainty that is only afforded by mass spectrometry (MS) detectors. We therefore thoroughly discuss the use of UHPLC-MS and UHPLC-tandem MS with regard to DMPK, highlighting the coupling of UHPLC with quadrupole-based MS analyzers, time-of-flight MS analyzers and hybrid analyzers. (C) 2014 Elsevier B.V. All rights reserved.
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