Journal
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
Volume 63, Issue -, Pages 44-54Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.trac.2014.06.023
Keywords
Isopycnic plot; Kinetic plot; Mass spectrometry; Orthogonal selectivity; Sub-2 mu m particle; Supercritical fluid chromatography (SFC); UHPSFC-MS; Ultra-high-performance supercritical fluid chromatography (UHPSFC); Ultra-high-performance liquid chromatography (UHPLC); van Deemter representation
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The development of greener analytical techniques is a topic of great interest and in this context, supercritical fluid chromatography (SFC) is making an interesting comeback. The present review focuses on the latest developments of this technique. Improvements brought to the chromatographic systems and requirements to achieve full compatibility with columns packed with sub-2 mu m particles (UHPSFC) are discussed. A thorough kinetic evaluation made using van Deemter representations, isopycnic and kinetic plots shows that performance achieved with state-of-the-art UHPSFC hardware and columns is comparable to that obtained in UHPLC. Orthogonal selectivity compared to reversed phase LC (RPLC) and extended selectivity modulation possibilities achievable in supercritical conditions using different stationary phase chemistries are presented. Finally, current applications of UHPSFC and its different hyphenation possibilities including mass spectrometry (MS) are also reviewed, in the hope of helping chromatography users to have a new look on the possibilities offered by this technique. (C) 2014 Published by Elsevier B.V.
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