Journal
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
Volume 29, Issue 3, Pages 269-280Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.trac.2009.12.007
Keywords
Biomarker; Derivatization-based GC-MS; Experiment-derived variation; Gas chromatography; Mass spectrometry; Metabolic pathway; Metabolomics; Metabonomics; Multi-origination phenomena; Multi-peak phenomena
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Funding
- Life Sciences Institute
- NUS Environmental Research Institute (NERI), National University of Singapore
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Gas chromatography combined with mass spectrometry (GC-MS), in particular derivatization-based GC-MS, is one of the most commonly used analytical methods in metabolomics. However, experiment-derived variations and originations of chromatographic peaks are two fundamental issues that could influence interpretation of metabolic pathways. Here, we focus on some pitfalls and limitations, especially the commonly ignored multi-origination and multi-peak phenomena in derivatization-based GC-MS metabolomics. We use examples to illustrate that ignoring these two factors could lead to wrong interpretations of results and serious metabolic implications. We also discuss strategies to overcome some of these confounding factors. (C) 2010 Elsevier Ltd. All rights reserved.
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