Effect of two synthetic disulfide bond variants of a 13-mer toxin from Conus californicus on the transcription of pro-inflammatory cytokines induced by LPS

This study presents the effects of two synthetic disulfide bond variants of cal16b, a 13-mer Ca2+ channel blocker conotoxin, on pro- and anti-inflammatory cytokine gene transcription in the murine macrophage-like cell line J774A.1 stimulated with LPS. The globular form (cal16b_1) acted as an anti-inflammatory agent; in contrast, the ribbon disulfide variant (cal16b_2) had a pro-inflammatory effect. Our results suggest that the pro- and anti-inflammatory effects are mediated by the three-dimensional structure. (C) 2013 Elsevier Ltd. All rights reserved.