Journal
TOXICON
Volume 60, Issue 5, Pages 874-877Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.toxicon.2012.06.014
Keywords
Toxin; Neutralizing antibodies; B cell epitope; Phage display
Categories
Funding
- National Institutes of Health [AI097688, AI082210]
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Residues Y-91-T-116 of ricin toxin's enzymatic subunit (RTA) constitute an immunodominant loop-helix-loop motif that is the target of two potent toxin neutralizing monoclonal antibodies (mAbs). PB10 and R70. To define the exact epitope(s) recognized by these mAbs, we affinity enriched from a phage-displayed peptide library 12 mers that bound one or both of these mAbs. We report that PB10 recognizes a distinct but overlapping epitope with R70, in which residues Q(98), E-102, T-105, and H-106 are central to mAb recognition. (C) 2012 Elsevier Ltd. All rights reserved.
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