Journal
TOXICON
Volume 57, Issue 4, Pages 566-573Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.toxicon.2010.12.020
Keywords
Benthic cyanobacteria; New Zealand; Saxitoxin; Scytonema; Scytonema crispum; 16S rRNA
Categories
Funding
- Environmental Science and Research
- Biomolecular Interaction Centre at the University of Canterbury
- Brian Mason Trust
- Environment Canterbury
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Saxitoxins or paralytic shellfish poisons (PSP) are neurotoxins produced by some species of freshwater cyanobacteria and marine dinoflagellates. Samples collected from the metaphyton of a drinking-water supply's pre-treatment reservoir and a small eutrophic lake in New Zealand returned positive results when screened using a Jellett PSP Rapid Test Kit. The dominant species in the sample was identified as Scytonema cf. crispum. A non-axenic clonal culture (UCFS10) was isolated from the lake. The partial 16S rRNA gene sequence shared only a 91% or less sequence similarity with other Scytonema species, indicating that it is unlikely that this genus is monophyletic and that further in-depth phylogenetic re-evaluation is required. The sxtA gene, which is known to be involved in saxitoxin production, was detected in UCFS10. Saxitoxin concentrations were determined from the lake samples and from UCFS10 using pre-column oxidation high performance liquid chromatography with fluorescence detection. Saxitoxin was the only variant detected and this was found at concentrations of 65.6 mu g g(-1) dry weight in the lake sample and 119.4 mu g g(-1) dry weight or 1.3 pg cell(-1) in UCFS10. This is the first confirmation of a saxitoxin-producing species in New Zealand and the first report of saxitoxin production by a species of Scytonema. (C) 2011 Elsevier Ltd. All rights reserved.
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