Journal
TOXICOLOGY MECHANISMS AND METHODS
Volume 22, Issue 7, Pages 533-539Publisher
TAYLOR & FRANCIS LTD
DOI: 10.3109/15376516.2012.692109
Keywords
ROFA; chronic exposure; air pollution; cardiopulmonary oxidative stress
Categories
Funding
- Universidade Federal de Ciencias da Saude de Porto Alegre, Brazil
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior - CAPES
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico - CNPq
Ask authors/readers for more resources
The current study aimed to determine the role of oxidants in cardiac and pulmonary toxicities induced by chronic exposure to ROFA. Eighty Wistar rats were divided into four groups: G1 (10 mu L Saline), G2 (ROFA 50 mu g/10 mu L), G3 (ROFA 250 mu g/10 mu L) and G4 (ROFA 500 mu g/10 mu L). Rats received ROFA by nasotropic instillation for 90 days. After that, they were euthanized and bronchoalveolar lavage (BAL) was performed for total count of leukocytes, protein and lactate dehydrogenase (LDH) determinations. Lungs and heart were removed to measure lipid peroxidation (MDA), catalase (CAT) and superoxide dismutase (SOD) activity. BAL presented an increase in leukocytes count in G4 in comparison to the Saline group (p = 0.019). In lung, MDA level was not modified by ROFA, while CAT was higher in G4 when compared to all other groups (p = 0.013). In heart, G4 presented an increase in MDA (p = 0.016) and CAT (p = 0.027) levels in comparison to G1. The present study demonstrated cardiopulmonary oxidative changes after a chronic ROFA exposure. More specifically, the heart tissue seems to be more susceptible to oxidative effects of long-term exposure to ROFA than the lung.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available