Journal
TOXICOLOGY LETTERS
Volume 230, Issue 2, Pages 218-224Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2014.01.042
Keywords
Grape stem extracts; Polyphenols; Vitis vinifera; Anticancer activity
Categories
Funding
- European Union (European Social Fund - ESF)
- Greek National Funds through the Operational Program 'Education and Lifelong Learning' of the National Strategic Reference Framework (NSRF) - Research Funding Program: 'Heracleitus II
- European Social Fund
Ask authors/readers for more resources
major part of the wineries' wastes is composed of grape stems which are discarded mainly in open fields and cause environmental problems due mainly to their high polyphenolic content. The grape stem extracts' use as a source of high added value polyphenols presents great interest because this combines a profitable venture with environmental protection close to wine-producing zones. In the present study, at first, the Total Polyphenolic Content (TPC) and the polyphenolic composition of grape stem extracts from four different Greek Vitis vinifera varieties were determined by HPLC methods. Afterwards, the grape stem extracts were examined for their ability to inhibit growth of colon (HT29), breast (MCF-7 and MDA-MB-23), renal (786-0 and Caki-1) and thyroid (K1) cancer cells. The cancer cells were exposed to the extracts for 72 h and the effects on cell growth were evaluated using the SRB assay. The results indicated that all extracts inhibited cell proliferation, with IC50 values of 121-230 mu g/ml (MCF-7), 121-184 mu g/ml (MDA-MD-23), 175-309 mu g/ml (HT29), 159-314 mu g/ml (K1), 180-225 mu g/ml ( 786-0) and 134->400 mu g/ml (Caki-1). This is the first study presenting the inhibitory activity of grape stem extracts against growth of colon, breast, renal and thyroid cancer cells. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available