Journal
TOXICOLOGY LETTERS
Volume 226, Issue 2, Pages 140-149Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2014.01.021
Keywords
AElatoxin B1; MicroRNA; Hepatocellular carcinoma; High throughput sequencing
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Funding
- National Natural Science Foundation of China [31000961, 31172297]
- Program for New Century Excellent Talents in University [NCET-10-0010]
- Fujian Fund of the Natural Science Foundation [2010101068]
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Aflatoxin B1 is a potent carcinogen which can induce** hepatocellular carcinoma (HCC) in mammals. Though microRNAs are known to play important roles in tumorigenesis, the functional complexity of microRNAs in AFB1-induced hepatocellular tumorigenesis has not yet been elucidated. Here, we applied Illumina deep sequencing technology for high-throughput profiling of microRNA in rat liver tissue before and after treatment with aflatoxin B1. Analysis of mature miRNAs from different arms of pre-miRNAs allowed us to identify the predominant form of miRNA. We studied the differential expression profile of miRNAs in two libraries, identifying several cancer-related microRNAs which exhibit abnormal expression. KEGG analysis indicated that predicted target genes of differentially expressed miRNAs are involved in cancer-related pathways. Bioinformatic analysis predicted 16 potential novel miRNAs. Our work provides new insights at the miRNA level into AFB1-induced hepatic injury which may lead to HCC. (C) 2014 Published by Elsevier Ireland Ltd.
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