Journal
TOXICOLOGY LETTERS
Volume 198, Issue 2, Pages 282-288Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2010.07.011
Keywords
Neurotoxicity; GABA; Chloride homeostasis; Inhibitory postsynaptic current; Tributyltin
Categories
Funding
- Japan Society for the Promotion of Science [15590207]
- Grants-in-Aid for Scientific Research [15590207] Funding Source: KAKEN
Ask authors/readers for more resources
Tributyltin (TBT) has harmful effects on invertebrates. Reports indicate that intoxication of humans with organotin compounds could be associated with neurological symptoms such as epilepsy and amnesia; however, the toxicity mechanisms in mammals are unknown. TBT acts as a Cl(-)/OH(-) antiporter, and likely affects the GABAergic system by disturbing Cl(-) homeostasis. This study aimed to elucidate neurotoxic actions of TBT on mouse neocortical neurons during development. From 4 days in vitro (4 DIV) or 14 DIV in culture, cortical neurons were exposed to TBT continuously for 3 days. TBT-induced neuronal death at 30 nM during DIV 4-6, and at 50 nM during DIV 14-16. To further characterize this age-dependent cytotoxicity, miniature postsynaptic currents (mPSCs) were analyzed by whole-cell patch-clamp. The frequency of mPSCs was significantly reduced by treatment with 30 nM TBT during DIV 4-6, but not DIV 14-16. After TBT treatment during DIV 4-6, GABA(A) receptor-mediated reversal potentials (E(GABA)) were significantly shifted negatively. The TBT-induced E(GABA) shift and neuronal death were reversed by increment of extracellular Cl- concentration, suggesting that disruption of Cl(-) homeostasis underlies the disturbance of neuronal ontogeny induced by TBT. These data indicate that the TBT may affect synaptogenesis and neuronal survival, particularly in early development. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available