Journal
TOXICOLOGY LETTERS
Volume 178, Issue 2, Pages 83-87Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.toxlet.2008.02.005
Keywords
mycotoxin; trichothecene; toxicokinetics; deoxynivalenol
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Funding
- NIDDK NIH HHS [R01 DK058833-08, R01 DK058833] Funding Source: Medline
- NIEHS NIH HHS [R01 ES003358, R56 ES003358, ES 3358, R01 ES003358-21] Funding Source: Medline
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Deoxynivalenol (DON or vomitoxin) is a trichothecene mycotoxin commonly found in cereal grains that adversely affects growth and immune function in experimental animals. A competitive enzyme-linked immunosorbent assay (ELISA) was used to monitor the kinetics of distribution and clearance of DON in tissues of young adult B6C3F1 male mice that were orally administered 25 mg/kg bw of the toxin. DON was detectable from 5 min to 24 h in plasma, liver, spleen and brain and from 5 min to 8 h in heart and kidney. The highest DON plasma concentrations were observed within 5-15 min (12 mu g/mL) after dosing. There was rapid clearance following two-compartment kinetics (t(1/2)alpha = 20.4 min, t(1/2)beta = 11.8 h) with 5% and 2% maximum plasma DON concentrations remaining after 8 and 24 h, respectively. DON distribution and clearance kinetics in other tissues were similar to that of plasma. At 5 min, DON concentrations in mu g/g were 19.5 +/- 1.9 in liver, 7.6 +/- 0.5 in kidney, 7.3 +/- 0.8 in spleen, 6.8 +/- 0.9 in heart and 0.8 +/- 0.1 in the brain. DON recoveries in tissues by ELISA were comparable to a previous study that employed I H-DON and 25 mg/kg bw DON dose. The ELISA was further applicable to the detection of DON in plasma of mice exposed to the toxin via diet. This approach provides a simple strategy that can be used to answer relevant questions in rodents of how dose, species, age, gender, genetic background and route/duration of exposure impact DON uptake and clearance. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
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