Journal
TOXICOLOGY IN VITRO
Volume 26, Issue 6, Pages 924-929Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tiv.2012.04.010
Keywords
Flavonoids; Hemichrome; Hypochlorous acid; Methemoglobin; Oxyhemoglobin; Stopped-flow spectrophotometry
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Funding
- Ministry of Science and Higher Education of the Polish Republic [NN 312 158 034]
- TUL Internal Scholarship Fund
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Hypochlorous acid (HOCI), produced by activated neutrophils, is highly reactive oxidizing and chlorinating agent of biologically relevant molecules. Hemoglobin (Hb), present in large amounts inside red blood cells, is the main target for HOCI in these cells. In this work heme damage of hemoglobin induced by hypochlorous acid in the absence and presence of some popular dietary flavonoids, catechin, epigallocatechin gallate and quercetin has been studied by stopped-flow spectrophotometry. Hypochlorous acid, being in a large molar excess to hemoglobin, initiates modifications of the heme group of oxy- as well as of methemoglobin, which eventually leads to heme damage. Flavonoids present at concentrations comparable with that of hemoglobin inhibit these processes. The kinetics of the reactions of the investigated flavonoids with HOCI has been also studied and the rate constants of the order of 10(5) M-1 s(-1) have been found. It is concluded that under conditions used in this study the inhibition of Hb heme damage by flavonoids results from the competition of these compounds with hemoglobin towards HOCI and/or from the formation of Hb-flavonoid complex in which heme group is more resistant against HOCI-induced damage. (c) 2012 Elsevier Ltd. All rights reserved.
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