Journal
TOXICOLOGY IN VITRO
Volume 25, Issue 1, Pages 138-144Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tiv.2010.10.005
Keywords
Quercetin; Rutin; Oxidative stress; Oxygen radical absorbance capacity; HepG2 cells
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Funding
- Korean Government (MOEHRD) [KRF-2007-511-F00030]
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Protective effects of quercetin and rutin against oxidative stress were evaluated using in vitro and intracellular antioxidant assay. Quercetin showed higher peroxyl and hydroxyl radical-scavenging activity in a dose-dependent manner than did rutin in oxygen-radical absorbance capacity (ORAC). At 10 and 100 mu M, quercetin had higher metal-chelating activity than rutin carrying rutinose at position C-3 and was also more efficient than rutin in reducing intracellular oxidative stress caused by peroxyl radicals and Cu2+. The protective activities of 10 and 100 mu M quercetin against Cu2+-induced intracellular oxidation were 13.8% and 44.8%, respectively. Rutin showed no protective activity against Cu2+-induced oxidative stress. Quercetin showed significantly lower intracellular Cu2+-chelating activity than did 1,10-phenanthroline but offered greater protection from Cu2+-induced oxidative stress. Thus. quercetin may diffuse through the cell membrane more efficiently than rutin because quercetin does not carry rutinose, is hydrophilic, and reduces Cu2+-induced oxidative stress by scavenging radicals instead of chelating with metal ions. (C) 2010 Elsevier Ltd. All rights reserved.
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