Journal
TOXICOLOGY IN VITRO
Volume 23, Issue 4, Pages 748-753Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tiv.2009.03.008
Keywords
Cell culture; Hepatocyte; HepaRG; In vitro; Drug metabolism; Cytochrome P450
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Funding
- EEC [LIINTOP-STREP-037499]
- Finnish Technological Research Agency (TEKES)
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Practically all human hepatocyte cell lines are deficient in major cytochrome P450 (CYP)-related enzyme activities, making them unrepresentative of in vivo hepatocytes. We have used the recently developed HepaRG cell line to determine the spectrum of most important CYP enzyme activities involved in xenobiotic metabolism (CYP1A1/2. CYNA6, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP2E1. and CYP3A4) and the effect of the prototypical CYP-inducer phenobarbital and a panel of known CYP-selective inhibitors on these activities. Comparison of these activities was carried out with two human primary hepatocyte populations. We show that excluding CYP2A6 and CYP2E1, HepaRG cells express high functional levels of most of the major xenobiotic metabolising CYPs. These activities were found to be selectively inhibited and induced by prototypical CYP-selective inhibitors and inducer at comparable levels to primary hepatocytes. In conclusion, HepaRG cells may be a promising cell line for various applications, which currently employ hepatic subcellular preparations or cultured primary hepatocytes. (C) 2009 Elsevier Ltd. All rights reserved.
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