Journal
TOXICOLOGY
Volume 303, Issue 1, Pages 107-114Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.tox.2012.10.025
Keywords
Chlorogenic acid; Liver inflammation; Liver fibrosis; TLR4
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Funding
- National Natural Science Foundation of China [81200310, 81070328]
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Chlorogenic acid (CGA) is a type of polyphenol with anti-inflammatory, antioxidant activities. Our previous studies showed CGA could efficiently inhibit carbon tetrachloride (CCl4)-induced liver fibrosis in rats. However, the specific underlying mechanism remains unclear. The aim of this study is to investigate the effects of CGA on liver inflammation and fibrosis induced by CCl4 and whether they are related to inhibition of toll-like receptor 4 (TLR4) signaling pathway. Male Sprague-Dawley (SD) rats were administrated CCl4 together with or without CGA for 8 weeks. Histopathological and biochemical analyses were carried out. The mRNA and protein expression levels of proinflammatory and profibrotic mediators were detected by RT-PCR and Western blot, respectively. The levels of serum prointlammatory cytokines were detected by ELISA. CGA significantly attenuated CCl4-induced liver damage and symptoms of liver fibrosis, accompanied by reduced serum transaminase levels, collagen land at-smooth muscle actin (alpha-SMA) expression. As compared with the CCl4-treated group, the expression levels of TLR4, myeloid differentiation factor 88 (MyD88), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were reduced in the treatment group of CCl4 and CGA, whereas bone morphogenetic protein and activin membrane-bound inhibitor (Bambi) expression was increased. CGA also suppressed CCl4 induced nuclear factor-kappa B (NF-kappa B) activation. Moreover, the hepatic mRNA expression and serum levels of tumor necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6) and interleukin-1 beta (IL-1 beta) were significantly increased in CCl4-treated rats and attenuated by co-treatment with CGA. Our data indicate that CGA can efficiently inhibit CCl4-induced liver fibrosis in rats and the protective effect may be due to the inhibition of TLR4/MyD88/NF-kappa B signaling pathway. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
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