Journal
TOXICOLOGY
Volume 284, Issue 1-3, Pages 19-25Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.tox.2011.03.010
Keywords
Bilirubin; Excitatory postsynaptic current (EPSC); NMDA receptor; AMPA receptor; Spontaneous action potential
Categories
Funding
- Ministry of Science and Technology, China [2009CB526504]
- Science and Technology Commission Foundation of Shanghai City [08JC1412700]
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Hyperbilirubinemia is one of the most common clinical phenomena observed in human newborns. To achieve effective therapeutic treatment, numerous studies have been done to determine the molecular mechanisms of bilirubin-induced neuronal excitotoxicity. However, there is no conclusive evidence for the involvement of glutamatergic synaptic transmission in bilirubin-induced neuronal hyperexcitation and excitotoxicity. In the present study, using gramicidin-perforated patch-clamp techniques, spontaneous excitatory postsynaptic currents (sEPSCs) were recorded from lateral superior olive (LSO) neurons isolated from postnatal 11-14-day-old (P11-14) rats. The application of 3 mu M bilirubin increased the frequency, but not the amplitude, of sEPSCs. The action of bilirubin was tetrodotoxin (TTX)-insensitive, as bilirubin also increased the frequency, but not the amplitude, of mEPSCs. The amplitudes of GABA-activated (I-GABA) and glutamate-activated (I-glu) currents were not affected by bilirubin. Under, current-clamp conditions, no spontaneous action potentials were observed in control solution. However, the application of 3 mu M bilirubin for 4-6 min evoked a considerable rate of action-potential firing. The evoked firing was partially occluded by D,L-2-amino-5-phosphonovaleric acid (APV), an NMDA receptor antagonist, but completely inhibited by a combination of APV and 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline-2,3-dione(NBQX), an AMPA receptor antagonist. These results indicate that bilirubin facilitates presynaptic glutamate release, enhances glutamatergic synaptic transmission by activating postsynaptic AMPA and NMDA receptors, and leads to neuronal hyperexcitation. This study provides a better understanding of the mechanism of bilirubin-induced excitotoxicity and determines for the first time that both AMPA and NMDA receptors are likely involved in the excitotoxicity produced by bilirubin. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
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