Journal
TOXICOLOGY
Volume 253, Issue 1-3, Pages 131-136Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.tox.2008.09.004
Keywords
Carbon nanotubes; Fibrosis; Granuloma; Inflammation; Lung; Rat
Categories
Funding
- French Ministry for Ecology
- Inserm and Assistance Publique - Hopitaux de Paris (Contrat d'Interface)
Ask authors/readers for more resources
Several studies performed by intratracheal instillation showed that carbon nanotubes (CNT) induced pulmonary fibrosis, granulomas or inflammation. But, recently, two inhalation Studies did not observed Such pathological phenomena and suggest that granulomas Could be due to the instillation of unbreathable agglomerates. In a previous study, we have described a simple method (using albumin as dispersing agent) which produced Solutions containing more than 80% of agglomerate ol'breathable size. We report here results from intratracheal instillation of rats by 0, 1, 10 or 100 mu g of MWCNT dispersed with albumin. After 1, 7, 30, 90, and 180 days, inflammation, apoptosis, fibrosis, respiratory parameters and granuloma formation were assessed. Results obtained by plethysmography, soluble collagen quantification, qRT-PCR and luminex measurement of cytokines expression and histopathological observation showed only evidence of apoptosis of alveolar macrophages.These result underline the importance of controlling MWCNT agglomerate size when exposing animals, through appropriate dispersion methods. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available