Journal
TOXICOLOGY
Volume 249, Issue 1, Pages 1-10Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.tox.2008.03.019
Keywords
polycyclic aromatic hydrocarbons; benzo[a]pyrene; dibenzo[a,l]pyrene; diesel exhaust; coal tar; proteomic; two-dimensional electrophoresis
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Funding
- NCI NIH HHS [CA28825, R01 CA028825] Funding Source: Medline
- NIEHS NIH HHS [P30 ES000210] Funding Source: Medline
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Polycyclic aromatic hydrocarbon (PAH) DNA adducts have been associated with carcinogenesis, which is accompanied by multiple alterations in gene expression. We used two-dimensional electrophoresis to distinguish protein expression changes induced in MCF-7 cells by individual PAH (B[a]P and DB[a,l]P) and PAH mixtures (coal tar extract [SRM 1597] and diesel exhaust extract [SRM 1975]). Spots of interest were identified by MALDI-TOF-TOF Our results have shown alterations in the expression of heat-shock proteins, cytoskeletal proteins, DNA associated proteins, and glycolytic and mitochondrial proteins. The proteins that were universally altered in expression were actin cytoplasmic 1, tubulin alpha and myosin light chain alkali, cyclophilin B, and heterogeneous ribonucleoprotein B1 (a protein involved in access to telomerase and mRNA maturation). Additional proteins with altered expression include histone H2A.1, heat-shock protein 70-2, galectin-3, nucleoside diphosphate kinase, ATP synthase, and electron transfer flavoprotein. While sharing similarities, each PAH treatment exhibited a unique proteomic fingerprint. (C) 2008 Elsevier Ireland Ltd. All rights reserved.
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