Journal
TOPICS IN CATALYSIS
Volume 55, Issue 16-18, Pages 1146-1156Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s11244-012-9893-0
Keywords
Specific enzyme immobilization; Nanomaterials; Affinity tag; Click chemistry; Polyhistidine; Biotin; Non-covalent
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Nanotechnology-inspired biocatalysts have aroused much interest recently because nanomaterials can provide the upper limits on enzyme-efficiency-determining factors. Non-specific immobilization approaches often face problems of enzyme leaching, 3D structure change and mass transfer resistance. Here, we focus on the review of specific enzyme immobilization approaches and have separated them into non-covalent and covalent categories.
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