Journal
TISSUE ENGINEERING PART C-METHODS
Volume 20, Issue 7, Pages 533-542Publisher
MARY ANN LIEBERT, INC
DOI: 10.1089/ten.tec.2013.0619
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Funding
- NIH/NIDCR [DE 13397-11]
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In the oral and craniofacial complex, tooth loss is the most commonly acquired disfiguring injury. Among the most formidable challenges of reconstructing tooth-supporting osseous defects in the oral cavity is the regeneration of functional multi-tissue complexes involving bone, ligament, and tooth cementum. Furthermore, periodontal multi-tissue engineering with spatiotemporal orientation of the periodontal ligament (PDL) remains the most challenging obstacle for restoration of physiological loading and homeostasis. We report on the ability of a hybrid computer-designed scaffold-developed utilizing computed tomography-to predictably facilitate the regeneration and integration of dental supporting tissues. Here, we provide the protocol for rapid proto-typing, manufacture, surgical implantation, and evaluation of dual-architecture scaffolds for controlling fiber orientation and facilitating morphogenesis of bone-ligament complexes. In contrast to conventional single-system methods of fibrous tissue formation, our protocol supports rigorous control of multi-compartmental scaffold architecture using computational scaffold design and manufacturing by 3D printing, as well as the evaluation of newly regenerated tissue physiology for clinical implementation.
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