Journal
TISSUE ENGINEERING PART C-METHODS
Volume 16, Issue 1, Pages 115-121Publisher
MARY ANN LIEBERT, INC
DOI: 10.1089/ten.tec.2008.0654
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Funding
- National Institutes of Health (NIH) [R21 EB005739-01]
- European Commission [EU STREP-CT-2005-018940]
- Federal Ministry of Education and Research, Germany [01GG0731/0313911]
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Spontaneous in vitro differentiation of mouse embryonic stem cells (mESC) is promoted by a dynamic, three-dimensional (3D), tissue-density perfusion technique with continuous medium perfusion and exchange in a novel four-compartment, interwoven capillary bioreactor. We compared ectodermal, endodermal, and mesodermal immunoreactive tissue structures formed by mESC at culture day 10 with mouse fetal tissue development at gestational day E9.5. The results show that the bioreactor cultures more closely resemble mouse fetal tissue development at gestational day E9.5 than control mESC cultured in Petri dishes.
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