Journal
TISSUE ENGINEERING PART A
Volume 16, Issue 5, Pages 1749-1759Publisher
MARY ANN LIEBERT, INC
DOI: 10.1089/ten.tea.2009.0650
Keywords
-
Categories
Funding
- Air Force Multidisciplinary University Research Initiative [F49620-021-0359]
- National Institute for Occupational Safety and Health/Centers for Disease Control and Prevention [200-2002-00528]
- National Science Foundation [CTS-0210238, DMI-9800565]
- National Institutes of Health [1 R01 EB004343, 1 R01 DE019430]
- Pennsylvania Department of Community and Economic Development
- Health Resources and Services Administration [1C76 HF 00381-01]
- National Tissue Engineering Center
- Department of Defense, the Scaife Foundation
- Philip and Marsha Dowd Engineering Seed Fund
- Plastic Surgery Education Foundation
- Cleft Palate Foundation
- American Society of Maxillofacial Surgeons
Ask authors/readers for more resources
The purpose of this study was to demonstrate spatial control of osteoblast differentiation in vitro and bone formation in vivo using inkjet bioprinting technology and to create three-dimensional persistent bio-ink patterns of bone morphogenetic protein-2 (BMP-2) and its modifiers immobilized within microporous scaffolds. Semicircular patterns of BMP-2 were printed within circular DermaMatrix (TM) human allograft scaffold constructs. The contralateral halves of the constructs were unprinted or printed with BMP-2 modifiers, including the BMP-2 inhibitor, noggin. Printed bio-ink pattern retention was validated using fluorescent or I-125-labeled bio-inks. Mouse C2C12 progenitor cells cultured on patterned constructs differentiated in a dose-dependent fashion toward an osteoblastic fate in register to BMP-2 patterns. The fidelity of spatial restriction of osteoblastic differentiation at the boundary between neighboring BMP-2 and noggin patterns improved in comparison with patterns without noggin. Acellular DermaMatrix constructs similarly patterned with BMP-2 and noggin were then implanted into a mouse calvarial defect model. Patterns of bone formation in vivo were comparable with patterned responses of osteoblastic differentiation in vitro. These results demonstrate that three-dimensional biopatterning of a growth factor and growth factor modifier within a construct can direct cell differentiation in vitro and tissue formation in vivo in register to printed patterns.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available